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Protein Purification

ProCrysta provides purification services for macromolecules such asenzymes,antibodies, hormones, Pegylated proteins, fusion proteins,peptidesetc. It can be done from pure form or crude sources like cell extract, fermentation media and clarified milk. These services are provided through its proprietaryprotein crystallization technology.

Through crystallization its offers several advantages like better purity and higher yields, uniform crystals size for therapeutic applications, takes less time, savings in global expenses and better formulation options with crystals over traditional purification methods.The process is reproducible and scalable to produce manufacturing scale batches under cGMP measures. Numerous advantages of protein crystallization include:

  • High purity and high yields: Crystallization process selectively crystallizes the protein of interest out of mixture of proteins and accomplishes purity and yields of proteins superior to conventional purification methods. Loss of protein is much less and reduces steps in purification process. Depending on protein, typical yields for crystallization is between 75-90% and the purity of the protein is above 90%.
  • Fewer steps and less time:  Crystallization process includes simple steps such as diafiltration, concentration of crude starting material, addition of crystallant and mixing in a vessel. Once crystallized crystals can be harvested and washed using centrifugation or TFF. The process time is optimized for 16-24 hours.
  • Simple instrumentation and reduced cost: Crystallization process can be performed in a closed environment, tank containing protein with overhead propeller and the crystallant. Most of the proteins crystallize at room temperature. Crystallization reduces the use of chromatography columns to very minimum and thus reduces the cost.
  • Storage options: Crystals are more stable at room temperature for longer time compared to soluble protein providing cost effective storage and longer self-life. Crystals can be stored in same crystallant until processed further for formulation.

    After reviewing protein properties and clients’ requirements purification methods are selected for the best outcome. Other purification techniques such as precipitation, affinity chromatography, Ion exchange chromatography, HPLC etc. are also used efficiently at ProCrysta.


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